Evaluation of a Minimally Destructive DNA Extraction Protocol in Canthon sp. (Coleoptera) and Oxytrigona sp. (Hymenoptera)
DOI:
https://doi.org/10.66778/RM.v09ed01.14Keywords:
Noninvasive, molecular identification, museum collections, Hymenoptera, Coleoptera, Lepidoptera, SDS, CTABAbstract
Insects constitute the most diverse group of animals on the planet, and their accurate identification is essential for research in conservation, agriculture, and taxonomy. In this context, DNA-based molecular techniques represent a highly valuable tool for their study; however, these tools are limited when working with specimens preserved in collections, as extraction protocols are often invasive and compromise the integrity of the insect body. The present study evaluated a minimally destructive DNA extraction protocol in two genera: Canthon sp. (Coleoptera) and Oxytrigona sp. (Hymenoptera). A total of 20 specimens were processed using a modification of the method described by Paydar et al. (2018). DNA quality was determined by spectrophotometry, measuring concentration and purity ratios A260/A280 and A260/A230. The results showed a markedly superior performance in Canthon sp., with concentrations of up to 563.2 ng/μl and purity ratios within the reference values, whereas Oxytrigona sp. yielded much lower concentrations (<3 ng/μl in most cases) and inadequate purity values. These differences can be attributed both to exoskeleton composition and to buffer formulation, as noted in previous studies. In conclusion, the adapted protocol proves effective for beetles, offering a non-destructive alternative for the molecular analysis of collection specimens, although adjustments are required for its application in hymenopterans.
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